An efficacy study was performed using a UVC and Ozone-generating device, the PathO3Gen Solutions’ Shoe Sanitizing Station, against Escherichia coli ( E. Coli), Pseudomonas aeruginosa, Methicillin-resistant Staphylococcus aureus ( MRSA Superbug), Vancomycin-resistant Enterococcus faecalis (VRE), Carbapenem-resistant Klebsiella pneumoniae, Candida auris, Aspergillusbrasiliensis, and Clostriodioides difficile (C. diff) and log and percent reduciton were quantified for each microorganism at three exposure times: 6, 8,and 10 seconds.
The main motivation of this study was to find the effectiveness of the patented Ozone + UVC device to destroy eight of the most common/severe bacterial, fungi, spore infections that affect healthcare facilities and spread over surfaces such as floors. At three different time intervals to find the efficacy at each interval.
Each microorganism was individually inoculated on separate Petri dishes and incubated at appropriate temperatures for a specific time period to allow for growth and colonization. Control samples were taken as well for all.
After the preparation of the microorganisms, a shoe sole (hard rubber substitute) was inoculated with the bacteria and then placed onto the sterilized shoe sanitizing station with a 150 lb. volunteer on top of the shoe with a sterile barrier between them.
The shoe and volunteer remained on the sanitizing station for each designated testing time period of 6, 8, and 10 seconds. Three tests were completed per microorganism, and time period. From the hard rubber substitutes inoculated with bacteria, two samples were taken from the shoe and placed in a petri dish and incubated for the appropriate lengths of time at specific temperatures.
After incubation, the viable microorganism colonies were counted, and data recorded. From the sample and control dishes, log and percent reductions were calculated.
The results from this study showed that for every type of bacteria, fungi, and spore tested, a reduction of at least 90% was seen. Meaning that from the viable bacteria which infected the sole of a shoe, the Shoe Sanitizing Station was able to destroy, at its lowest time interval and toughest organism, 90% of the microorganism. It kills Candida auris at 5.16 log, or 99.999% in 10 seconds, which is very difficult and unheard of from any other product.